Dr. Md. Hasanur Alam
Dr. Md. Hasanur Alam

Assistant Professor Contact No : +88 01723888223
Department of Animal Science Email : hasanur@bau.edu.bd
Faculty of Animal Husbandry ResearchGate
 
Research Interest:   Animal Production, Reproductive Biotechnology, Oocyte
PhD    - 2018
Kobe University
Japan
MS    - 2013
Bangladesh Agricultural University
Bangladesh
B.Sc. (Honours)    - 2012
Bangladesh Agricultural University
Bangladesh
Total Number:  6
 

Research Achievements Bovine oocytes increase in diameter from 30 µm to 125 µm, and an antrum is formed inside the follicles. Bovine growing oocytes of >100 µm are able to resume meiosis, although they are incompetent to mature to metaphase II. Thus, their precocious resumption of meiosis during growth culture makes it difficult for them to grow to full meiotic competent oocytes.

i) PDE3 inhibitors induced formation of antrum-like structures by oocyte-granulosa cell complexes (OGCs) and maintained meiotic arrest of oocytes during culture. Since formation of the antrum-like structures was promoted with the inhibition of PDE3, which is functional only in oocytes, I thought that the PDE3 inhibitors might promote expression of some oocytes-derived factors, which in turn induce the formation of antrum-like structures.

ii) OGCs developed antrum-like structures, whereas oocytectomized complexes (OXCs) and granulosa cell complexes (GCs) did not develop the structure. However, GDF9 and BMP15 induced formation of antrum-like structures in OXCs and GCs. To complete the hypothesis, I examined the effect of PDE inhibitors on the expression of GDF9 and BMP15 mRNAs in in vitro grown bovine oocytes.

iii) The relative mRNA levels of GDF9 and BMP15 increased in the oocytes after culture with PDE3 inhibitors.

From these studies, I found that inhibition of oocyte PDE3 maintained meiotic arrest of oocytes and increased expression of GDF9 and BMP15 mRNAs. The oocytes induce formation of antrum-like structures by the granulosa cells via oocyte-derived factors, GDF9 and BMP15.

No training information available..
Assistant Professor
15 Feb, 2017 -
Bangladesh Agricultural University
Lecturer
15 Feb, 2015 - 14 Feb, 2017
Bangladesh Agricultural University
Total Number:  11
 

List of Publications

  1. Alam MH, Lee J, Miyano T. Interaction between growing oocytes and granulosa cells in vitro. Reproductive Biology and medicine. Invited review (submitted).
  2. Alam MH, Lee J, Miyano T. GDF9 and BMP15 induce development of antrum-like structures by bovine granulosa cells without oocytes. Journal of Reproduction and Development, 64, 5, 423-431, 2018.
  3. Alam MH, Lee J, Miyano T. Inhibition of PDE3A sustains meiotic arrest and gap junction of bovine growing oocytes in in vitro growth culture. Theriogenology, 118, 110-118, 09, 2018.
  4. Sarker MB, Alam MH, Saha BK, Amin MR, Moniruzzaman M. Effects of soybean milk replacer on growth, meat quality, rumen and gonad development of goats. Small Ruminant Research, 130, 127-135, 07, 2015.
  5. Alam MH, Kabir ME, Sarker MB, Saha BK, Khan RI, Moniruzzaman M. Development and survivability of ovarian follicles of goat in different feeding systems. Bangladesh Journal of Animal Science, 43, 3, 175-179, 12, 2014.
  6. Saha BK, Sarker MB, Alam MH, Kabir ME, Amin MR, Moniruzzaman M. Effect of remating intervals on growth and reproductive performance of rabbits. Bangladesh Journal of Animal Science, 42, 2, 143-147, 12, 2013.

List of Proceedings

  1. Islam MN, Alam MH, Khatun A, Hashem MA, Moniruzzaman M. Kit ligand enhances growth and nuclear maturation of buffalo oocytes in vitro. 45th IETS Annual Conference, New Orleans, USA.
  2. Alam MH, Lee J, Miyano T. GDF9 and BMP15 promote antrum formation by bovine cumulus cells in vitro. The 124th JSAS Annual Meeting, Tokyo, Japan.
  3. Alam MH, Lee J, Miyano T. Effect of PDE inhibitors on growth and maturational competence of partially meiotic-competent bovine oocytes in vitro. 4 th World Congress Of Reproductive Biology, Okinawa, Japan.
  4. Sarker MB, Alam MH, Saha BK, Amin MR, Moniruzzaman M. Effects of feeding soymilk based milk replacer on growth and rumen development in kids. Proceedings of the 30th Biennial Conference of the Australian Society of Animal Production, Canberra, Australia.
  5. Islam MM, Sarker MB, Alam MH, Khan RI, Moniruzzaman M. Germination test of wheat for pregnancy diagnosis of goats and sheep. Proceedings of the 16th AAAP Animal Science Congress Vol. II, Yogyakarta, Indonesia.
No participation information available..
No patent information available..
Title Institution Subject Receipt Date
2nd best poster presenter BAHA Poster presentation 2013
MEXT Scholarship Japan Government PhD Fellow 2014
Membership Type Organization
Student member The Society for Reproduction and Development